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A novel mcl PHA-producing bacterium, Pseudomonas guezennei sp nov, isolated from a 'kopara' mat located in Rangiroa, an atoll of French Polynesia ArchiMer
Simon-colin, Christelle; Alain, Karine; Colin, Sebastien; Cozien, Joelle; Costa, B; Guezennec, Jean; Raguenes, Gerard.
Aims: The aim of the present study was to describe an aerobic, mesophilic and heterotrophic bacterium, designated RA26, able to produce a medium-chain-length polyhydroxyalkanoate (PHA). It was isolated from a French Polynesian bacterial mat located in the atoll of Rangiroa. Methods and Results: This micro-organism, on the basis of the phenotypical features and genotypic investigations can be clearly assigned to the Pseudomonas genus and the name of Pseudomonas guezennei is proposed. Optimal growth occurs between 33 and 37 degrees C, at a pH between 6 4 and 7 1 and at ionic strength of 15 g 1(-1) of sea salts. The G+C content of DNA is 63.2%. Under laboratory conditions, this bacterium produced a novel, medium-chain-length PHA, mainly composed of...
Tipo: Text Palavras-chave: Pseudomonas; Polyhydroxyalkanaote; Microbial mat; Kopara.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-3858.pdf
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Exopolysaccharide biosynthesis and biodegradation by a marine hydrothermal Alteromonas sp. strain ArchiMer
Lelchat, Florian; Cozien, Joelle; Le Costaouec, Tinaig; Brandily, Christophe; Schmitt, Sophie; Baudoux, Anne-claire; Colliec-jouault, Sylvia; Boisset, Claire.
Alteromonas macleodii subsp. fijiensis biovar deepsane is a deep-sea ecotype exopolysaccharide-producing bacteria isolated from the polychaete annelid Alvinella pompejana. The high molecular weight biopolymer HYD657 produced by this strain, is the first marine exopolysaccharide (EPS) to be commercialized for cosmetic use. Depolymerization methods are necessary to elucidate the complete structure of this EPS and to generate potentially bioactive oligosaccharides. Enzymatic methods are useful for elucidating polysaccharide structure because they specifically cleave glycosidic bonds and do not require harsh chemical conditions. The HYD657 EPS is structurally complex and no commercially available enzymes are able to effectively degrade it. Here, we present the...
Tipo: Text Palavras-chave: Marine Alteromonas macleodii sp strain; Bacterial exopolysaccharides; Microbial extracellular enzymes; Endogenous enzymatic degradation.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00240/35129/33893.pdf
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Molecular evolution and phylogeography of infectious hematopoietic necrosis virus with a focus on its presence in France over the last 30 years ArchiMer
Bellec, Laure; Louboutin, Lenaig; Cabon, Joelle; Castric, Jeanne; Cozien, Joelle; Thiery, Richard; Morin, Thierry.
Infectious hematopoietic necrosis virus (IHNV) is among the most important pathogens affecting the salmonid industry. Here, we investigated the molecular evolution and circulation of isolates from 11 countries or regions all over the world, with a special focus on the epidemiological situation in France. The phylogeography, time to the most recent common ancestor (TMRCA) and nucleotide substitution rate were studied using 118 full-length glycoprotein gene sequences isolated from 9 countries (5 genogroups) over a period of 47 years. The TMRCA dates back to 1943, with the L genogroup identified as the likely root (67 %), which is consistent with the first report of this pathogen in the USA. A Bayesian inference approach was applied to the partial...
Tipo: Text Palavras-chave: Infectious hematopoietic necrosis virus; Evolutionary dynamics; Dates of divergence; Phylogeography; Molecular epidemiology.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00399/51015/51832.pdf
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Occurrence of Bacterial Pathogens and Human Noroviruses in Shellfish-Harvesting Areas and Their Catchments in France ArchiMer
Rince, Alain; Baliere, Charlotte; Hervio-heath, Dominique; Cozien, Joelle; Lozach, Solen; Parnaudeau, Sylvain; Le Guyader, Francoise S.; Le Hello, Simon; Giard, Jean-christophe; Sauvageot, Nicolas; Benachour, Abdellah; Strubbia, Sofia; Gourmelon, Michele.
During a 2-year study, the presence of human pathogenic bacteria and noroviruses was investigated in shellfish, seawater and/or surface sediments collected from three French coastal shellfish-harvesting areas as well as in freshwaters from the corresponding upstream catchments. Bacteria isolated from these samples were further analyzed. Escherichia coli isolates classified into the phylogenetic groups B2, or D and enterococci from Enterococcus faecalis and E. faecium species were tested for the presence of virulence genes and for antimicrobial susceptibility. Salmonella members were serotyped and the most abundant serovars (Typhimurium and its monophasic variants and Mbandaka) were genetically characterized by high discriminative subtyping methods....
Tipo: Text Palavras-chave: Campylobacter; Salmonella; Vibrio; HuNoVs; Fecal bacterial indicators; Shellfish; Water.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00461/57243/59243.pdf
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